午夜高清影院免费观看,亚洲欧美国产第一区第二区,亚洲精品午夜久久久,无遮挡又爽又刺激的视频

免費(fèi)咨詢熱線:
技術(shù)文章
首頁(yè) > 技術(shù)中心 > PEI25K轉(zhuǎn)染試劑的應(yīng)用

PEI25K轉(zhuǎn)染試劑的應(yīng)用

 更新時(shí)間:2022-11-24 點(diǎn)擊量:1295

PEI又稱為線性化聚乙烯亞胺,是人工合成的含有高密度正電荷的有機(jī)大分子陽(yáng)離子聚合物。PEIDNA通過(guò)正負(fù)電荷的相互吸引形成復(fù)合物,這些復(fù)合物很容易通過(guò)內(nèi)吞作用進(jìn)入細(xì)胞。PEI-DNA復(fù)合物能很好地抵御溶酶體的降解作用,有效提高轉(zhuǎn)染效率。PEI可作用于大至135000個(gè)核苷酸的質(zhì)粒,所以較大的質(zhì)粒也可以成功轉(zhuǎn)染。目前市面上PEI轉(zhuǎn)染試劑有很多種,PolysciencesPEI線性轉(zhuǎn)染試劑適用于多種真核細(xì)胞,用途廣泛。

一、Polysciences線性PEI轉(zhuǎn)染試劑(PEI 25K)特性:

貨號(hào):23966-1

分子式:(CH2CH2NH)n

分子量:25000

外觀:白色至黃色粉末

熔點(diǎn):73-75

溶解性:溶于:熱水、低pH值的冷水、甲醇和乙醇;

不溶于:苯、YI醚和丙酮。

微信截圖_20221123145305.png

二、Polysciences聚乙烯亞胺轉(zhuǎn)染試劑(PEI 25K)的應(yīng)用:

遞送物質(zhì)
轉(zhuǎn)染細(xì)胞類型轉(zhuǎn)染細(xì)胞舉例(包括但不限于)
DNA貼壁/懸浮細(xì)胞HEK293
CHO
HELA
SF9昆蟲(chóng)細(xì)胞
SF21昆蟲(chóng)細(xì)胞等真核細(xì)胞系
三、應(yīng)用Polysciences PEI線性轉(zhuǎn)染試劑發(fā)表的高分文章:

(1)Y. Durocher, et al. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research, 2022, 30(2), e9-e9.  IF19.160

試劑:PEI 25K;應(yīng)用:DNA轉(zhuǎn)染人胚腎293S細(xì)胞。

(2)S. Siegman, et al. Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia, 2015, 28, 45-54.  IF10.633

試劑:PEI 25K;應(yīng)用:質(zhì)粒DNA轉(zhuǎn)染小鼠骨髓間充質(zhì)干細(xì)胞(D1)。

(3)M. P. Trefny, et al. PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade. Cancer Immunology, Immunotherapy: CII., 2020.  IF6.630

試劑:PEI 25K;應(yīng)用:包裝質(zhì)粒pCMVdelta8.9、包膜質(zhì)粒VSV-GPD-1-GFP、PD-L1-GFP或空GFP sFFV轉(zhuǎn)移質(zhì)粒轉(zhuǎn)染HEK293T細(xì)胞

(4)C. Schmitz, et al. Live reporting for hypoxia: Hypoxia sensormodified mesenchymal stem cells as in vitro reporters. Biotechnology and Bioengineering, 2020, 117(11), 3265-3276.  IF4.395

試劑:PEI 25K;應(yīng)用:轉(zhuǎn)染HEK293T細(xì)胞。

(5)J. Li, et al. Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 2022, 13, 3739.  IF:4.345

試劑:PEI 25K(23966-1)應(yīng)用:質(zhì)粒轉(zhuǎn)染HEK293FT細(xì)胞。

(62)S. Bang, et al. The Clinical Relevance and Function of Krüppel-Like Factor 16 in Breast Cancer. Cancer Management and Research, 2020, 12, 6373.  IF3.602

試劑:PEI 25K(23966-1);應(yīng)用:shRNA-PLKO.1質(zhì)粒轉(zhuǎn)染HEK293FT細(xì)胞

(7)S. Wulhfard, et al. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 2010, 148(2-3), 128-132.  IF3.595

試劑:PEI 25K;應(yīng)用:質(zhì)粒DNA轉(zhuǎn)染懸浮生長(zhǎng)的CHO DG44細(xì)胞。

(8)A. Sanzhez-Mata, et al. Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR protocols, 2020, 1(3), 100183.

試劑:PEI 25K;應(yīng)用:DNA轉(zhuǎn)染HEK293T細(xì)胞。

(9)M. Thomas, et al. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proceedings of the National Academy of Sciences, 2005, 102(16), 5679-5684.  

試劑:PEI 25K應(yīng)用:將編碼fLuc基因的DNA遞送至小鼠。

(10)L. Baranyi, et al. Rapid generation of stable cell lines expressing high levels of erythropoietin, factor VIII, and an antihuman CD20 antibody using lentiviral vectors. Human Gene Therapy Methods, 2013, 24(4), 214-227.  

試劑:PEI MAX(24765);應(yīng)用:用質(zhì)粒DNA轉(zhuǎn)染ET801載體—幼倉(cāng)鼠腎來(lái)源細(xì)胞。

北京百奧創(chuàng)新科技有限公司提供多款Polysciences轉(zhuǎn)染試劑,具體信息請(qǐng)咨詢百奧創(chuàng)新客服。(Polysciences PEI 25K 23966-1現(xiàn)貨打折,*!機(jī)會(huì)有限,不要錯(cuò)過(guò)!)

微信截圖_20221123150211.png



亚洲中文字幕无码一区| 亚洲欧美日韩在线三区| 欧美大片在线免费看精品一区| 亚洲AV综合色一区二区三区| 色婷婷是五月综合色| 婷婷激情网五月天亚洲| 人妻熟女的高跟丝袜艳遇| 欧美日韩精品一区二区三区激情在线| 日本午夜免费福利视频| 亚洲影视一区二区三区| 花花草草寻亲记哪里看全集| 亚洲欧美另类久久久精品| 人妻精品久久一区二区| 亚洲最大国产精品一区| 欧美黑粗大长在线不卡| 亚洲国产精品国揄产拍| 欧美午夜激情久久久久| 那个网站可以看理论片| 日韩欧美精品在线中文字幕| 国产盗摄精品一区二区视频| 9久久99久久久免费精品然| 2020国自产拍精品网站| 欧美日本大陆一区二区| 国产精品青青青高清在线观看| 波多野结衣人妻奴隶| 日韩精品视频在线观看一区二区三区| 一区二区日韩av激情| 成人一级片黄色一级片| 欧美精品在欧美一区二区三区| 日本在线一区二区免费| 欧美日本一道本一区二区三区| 女人和男人三级黄片子| 波多野结衣av一区二区| 欧美成人3p视频在线观看| 东北乱国产对白刺激视频| 国产亚洲综合性久久影院| 日韩精品视频在线观看一区二区三区| 久久久久久久久久久极品| 韩国三级电影视频网站| 亚洲人妻御姐中文字幕| 三点水一个色是什么字|